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- Analysis report

Date: 2021-04-22

Submitted by: Anonymous

Analysis type: Class I

Description of experiment:

None provided

Alleles: H-2-Db, H-2-Kb

Samples:

mouse_liver.txt

Sample Overview

Peptide Length Distribution (maximum of 30 mers)

Sample Peptide length Total peptides %
mouse_liver.txt all lengths 983 100
8-12 mers 885 90
other 98 10

Annotation Results

NetMHCpan eluted ligand predictions made for all peptides between 8 & 12 mers, inclusive. Percent rank cutoffs for strong and weak binders: 0.5 and 2.0.

Allele Sample Total peptides Strong binders Weak binders Non-binders

H-2-Db

 mouse_liver.txt 885 250 (28.2%) 82 (9.3%) 553 (62.5%)

H-2-Kb

 mouse_liver.txt 885 317 (35.8%) 105 (11.9%) 463 (52.3%)
Binding Affinities

Binding Heatmaps

NetMHCpan eluted ligand predictions made for all peptides between 8 & 12 mers, inclusive.

Predicted strong binders

Predicted weak binders

Predicted non-binders

- - # of peptides in sample

Sequence Motifs

Clustering performed with all peptides between 8 & 12 mers, inclusive.

Percentages represent the percentage of peptides in a given group predicted to strongly bind the indicated allele.

Polar

Neutral

Basic

Acidic

Hydrophobic

mouse_liver.txt (peptides used: 885, outliers: 112)

Peptides in group: 390

H-2-Db: 2%,

H-2-Kb: 74%

Peptides in group: 383

H-2-Db: 63%,

H-2-Kb: 8%

mouse_liver.txt sequence motif(s)
H-2-Db

Peptides: 327

H-2-Kb

Peptides: 416

Non-binders group 2

Peptides: 65

Non-binders group 3

Peptides: 55

Non-binders group 4

Peptides: 70

Non-binders group 1

Peptides: 70